Coding

Part:BBa_K1175006:Design

Designed by: Nicholas K. Goldner   Group: iGEM13_WLC-Milwaukee   (2013-09-17)


endo-beta-1,3-1,4 glucanase (BglS) from Bacillus Subtilis 168


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

No design constraints were needed for RF10 insertion, a second stop codon was also added to the end of the coding region.


Source

Bacillus Subtilis 168

References